[protocol.begin "steponeplus" "SYBR Green RNA-to-Ct 1-Step with melt curve"]
<?xml version="1.0" encoding="UTF-8" standalone="yes"?>
<TCProtocol>
    <ProtocolName>SYBR Green RNA-to-Ct 1-Step with melt curve</ProtocolName>
    <UserName/>
    <BlockID>0</BlockID>
    <TubeType>0</TubeType>
    <SampleVolume>20.0</SampleVolume>
    <RunMode>Fast</RunMode>
    <CoverTemperature>105.0</CoverTemperature>
    <Delay>0.0</Delay>
    <ExtendedPCRCycles>0</ExtendedPCRCycles>
    <ExtendedHoldTemp>0</ExtendedHoldTemp>
    <ExtendedHoldTime>0</ExtendedHoldTime>
    <TCStage>
        <StageFlag>PRE_CYCLING</StageFlag>
        <NumOfRepetitions>1</NumOfRepetitions>
        <StartingCycle>0</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>48.0</Temperature>
            <HoldTime>1800</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>600</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
    </TCStage>
    <TCStage>
        <StageFlag>CYCLING</StageFlag>
        <NumOfRepetitions>40</NumOfRepetitions>
        <StartingCycle>1</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>15</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>1</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>60.0</Temperature>
            <HoldTime>60</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
    </TCStage>
    <TCStage>
        <StageFlag>DISSOCIATION</StageFlag>
        <NumOfRepetitions>1</NumOfRepetitions>
        <StartingCycle>0</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>15</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>60.0</Temperature>
            <HoldTime>15</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>1</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>15</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
            <CollectionMode>StepAndHold</CollectionMode>
            <Type>Dissociation</Type>
            <DeltaTemperature>0.3</DeltaTemperature>
        </TCStep>
    </TCStage>
</TCProtocol>

[protocol.end]

[protocol.begin "steponeplus" "SYBR Green RNA-to-Ct 1-Step without melt curve"]
<?xml version="1.0" encoding="UTF-8" standalone="yes"?>
<TCProtocol>
    <ProtocolName>SYBR Green RNA-to-Ct 1-Step without melt curve</ProtocolName>
    <UserName/>
    <BlockID>0</BlockID>
    <TubeType>0</TubeType>
    <SampleVolume>20.0</SampleVolume>
    <RunMode>Fast</RunMode>
    <CoverTemperature>105.0</CoverTemperature>
    <Delay>0.0</Delay>
    <ExtendedPCRCycles>0</ExtendedPCRCycles>
    <ExtendedHoldTemp>0</ExtendedHoldTemp>
    <ExtendedHoldTime>0</ExtendedHoldTime>
    <TCStage>
        <StageFlag>PRE_CYCLING</StageFlag>
        <NumOfRepetitions>1</NumOfRepetitions>
        <StartingCycle>0</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>48.0</Temperature>
            <HoldTime>1800</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>600</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
    </TCStage>
    <TCStage>
        <StageFlag>CYCLING</StageFlag>
        <NumOfRepetitions>40</NumOfRepetitions>
        <StartingCycle>1</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>15</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>1</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>60.0</Temperature>
            <HoldTime>60</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
    </TCStage>
</TCProtocol>

[protocol.end]


[protocol.begin "steponeplus" "TaqMan RNA-to-Ct 1-Step"]
<?xml version="1.0" encoding="UTF-8" standalone="yes"?>
<TCProtocol>
    <ProtocolName>TaqMan RNA-to-Ct 1-Step</ProtocolName>
    <UserName/>
    <BlockID>0</BlockID>
    <TubeType>0</TubeType>
    <SampleVolume>20.0</SampleVolume>
    <RunMode>Fast</RunMode>
    <CoverTemperature>105.0</CoverTemperature>
    <Delay>0.0</Delay>
    <ExtendedPCRCycles>0</ExtendedPCRCycles>
    <ExtendedHoldTemp>0</ExtendedHoldTemp>
    <ExtendedHoldTime>0</ExtendedHoldTime>
    <TCStage>
        <StageFlag>PRE_CYCLING</StageFlag>
        <NumOfRepetitions>1</NumOfRepetitions>
        <StartingCycle>0</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>48.0</Temperature>
            <HoldTime>900</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>600</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
    </TCStage>
    <TCStage>
        <StageFlag>CYCLING</StageFlag>
        <NumOfRepetitions>40</NumOfRepetitions>
        <StartingCycle>1</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>15</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>1</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>60.0</Temperature>
            <HoldTime>60</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
    </TCStage>
</TCProtocol>

[protocol.end]

[protocol.begin "steponeplus" "Fast SYBR Green with melt curve"]
<?xml version="1.0" encoding="UTF-8" standalone="yes"?>
<TCProtocol>
    <ProtocolName>Fast SYBR Green with melt curve</ProtocolName>
    <UserName/>
    <BlockID>0</BlockID>
    <TubeType>0</TubeType>
    <SampleVolume>20.0</SampleVolume>
    <RunMode>Fast</RunMode>
    <CoverTemperature>105.0</CoverTemperature>
    <Delay>0.0</Delay>
    <ExtendedPCRCycles>0</ExtendedPCRCycles>
    <ExtendedHoldTemp>0</ExtendedHoldTemp>
    <ExtendedHoldTime>0</ExtendedHoldTime>
    <TCStage>
        <StageFlag>PRE_CYCLING</StageFlag>
        <NumOfRepetitions>1</NumOfRepetitions>
        <StartingCycle>0</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>20</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
    </TCStage>
    <TCStage>
        <StageFlag>CYCLING</StageFlag>
        <NumOfRepetitions>40</NumOfRepetitions>
        <StartingCycle>0</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>3</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>1</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>60.0</Temperature>
            <HoldTime>30</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
    </TCStage>
    <TCStage>
        <StageFlag>DISSOCIATION</StageFlag>
        <NumOfRepetitions>1</NumOfRepetitions>
        <StartingCycle>0</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>15</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>60.0</Temperature>
            <HoldTime>60</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>1</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>15</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
            <CollectionMode>StepAndHold</CollectionMode>
            <Type>Dissociation</Type>
            <DeltaTemperature>0.3</DeltaTemperature>
        </TCStep>
    </TCStage>
</TCProtocol>

[protocol.end]


[protocol.begin "steponeplus" "Fast SYBR Green without melt curve"]
<?xml version="1.0" encoding="UTF-8" standalone="yes"?>
<TCProtocol>
    <ProtocolName>Fast SYBR Green without melt curve</ProtocolName>
    <UserName/>
    <BlockID>0</BlockID>
    <TubeType>0</TubeType>
    <SampleVolume>20.0</SampleVolume>
    <RunMode>Fast</RunMode>
    <CoverTemperature>105.0</CoverTemperature>
    <Delay>0.0</Delay>
    <ExtendedPCRCycles>0</ExtendedPCRCycles>
    <ExtendedHoldTemp>0</ExtendedHoldTemp>
    <ExtendedHoldTime>0</ExtendedHoldTime>
    <TCStage>
        <StageFlag>PRE_CYCLING</StageFlag>
        <NumOfRepetitions>1</NumOfRepetitions>
        <StartingCycle>0</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>20</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
    </TCStage>
    <TCStage>
        <StageFlag>CYCLING</StageFlag>
        <NumOfRepetitions>40</NumOfRepetitions>
        <StartingCycle>0</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>3</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>1</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>60.0</Temperature>
            <HoldTime>30</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
    </TCStage>
</TCProtocol>

[protocol.end]


[protocol.begin "stepone" "SYBR Green RNA-to-Ct 1-Step with melt curve"]
<?xml version="1.0" encoding="UTF-8" standalone="yes"?>
<TCProtocol>
    <ProtocolName>SYBR Green RNA-to-Ct 1-Step with melt curve</ProtocolName>
    <UserName/>
    <BlockID>0</BlockID>
    <TubeType>0</TubeType>
    <SampleVolume>20.0</SampleVolume>
    <RunMode>Fast</RunMode>
    <CoverTemperature>105.0</CoverTemperature>
    <Delay>0.0</Delay>
    <ExtendedPCRCycles>0</ExtendedPCRCycles>
    <ExtendedHoldTemp>0</ExtendedHoldTemp>
    <ExtendedHoldTime>0</ExtendedHoldTime>
    <TCStage>
        <StageFlag>PRE_CYCLING</StageFlag>
        <NumOfRepetitions>1</NumOfRepetitions>
        <StartingCycle>0</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>48.0</Temperature>
            <HoldTime>1800</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>600</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
    </TCStage>
    <TCStage>
        <StageFlag>CYCLING</StageFlag>
        <NumOfRepetitions>40</NumOfRepetitions>
        <StartingCycle>1</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>15</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>1</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>60.0</Temperature>
            <HoldTime>60</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
    </TCStage>
    <TCStage>
        <StageFlag>DISSOCIATION</StageFlag>
        <NumOfRepetitions>1</NumOfRepetitions>
        <StartingCycle>0</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>15</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>60.0</Temperature>
            <HoldTime>15</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>1</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>15</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
            <CollectionMode>StepAndHold</CollectionMode>
            <Type>Dissociation</Type>
            <DeltaTemperature>0.3</DeltaTemperature>
        </TCStep>
    </TCStage>
</TCProtocol>

[protocol.end]


[protocol.begin "stepone" "SYBR Green RNA-to-Ct 1-Step without melt curve"]
<?xml version="1.0" encoding="UTF-8" standalone="yes"?>
<TCProtocol>
    <ProtocolName>SYBR Green RNA-to-Ct 1-Step without melt curve</ProtocolName>
    <UserName/>
    <BlockID>0</BlockID>
    <TubeType>0</TubeType>
    <SampleVolume>20.0</SampleVolume>
    <RunMode>Fast</RunMode>
    <CoverTemperature>105.0</CoverTemperature>
    <Delay>0.0</Delay>
    <ExtendedPCRCycles>0</ExtendedPCRCycles>
    <ExtendedHoldTemp>0</ExtendedHoldTemp>
    <ExtendedHoldTime>0</ExtendedHoldTime>
    <TCStage>
        <StageFlag>PRE_CYCLING</StageFlag>
        <NumOfRepetitions>1</NumOfRepetitions>
        <StartingCycle>0</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>48.0</Temperature>
            <HoldTime>1800</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>600</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
    </TCStage>
    <TCStage>
        <StageFlag>CYCLING</StageFlag>
        <NumOfRepetitions>40</NumOfRepetitions>
        <StartingCycle>1</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>15</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>1</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>60.0</Temperature>
            <HoldTime>60</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
    </TCStage>
</TCProtocol>

[protocol.end]

[protocol.begin "stepone" "TaqMan RNA-to-Ct 1-Step"]
<?xml version="1.0" encoding="UTF-8" standalone="yes"?>
<TCProtocol>
    <ProtocolName>TaqMan RNA-to-Ct 1-Step</ProtocolName>
    <UserName/>
    <BlockID>0</BlockID>
    <TubeType>0</TubeType>
    <SampleVolume>20.0</SampleVolume>
    <RunMode>Fast</RunMode>
    <CoverTemperature>105.0</CoverTemperature>
    <Delay>0.0</Delay>
    <ExtendedPCRCycles>0</ExtendedPCRCycles>
    <ExtendedHoldTemp>0</ExtendedHoldTemp>
    <ExtendedHoldTime>0</ExtendedHoldTime>
    <TCStage>
        <StageFlag>PRE_CYCLING</StageFlag>
        <NumOfRepetitions>1</NumOfRepetitions>
        <StartingCycle>0</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>48.0</Temperature>
            <HoldTime>900</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>600</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
    </TCStage>
    <TCStage>
        <StageFlag>CYCLING</StageFlag>
        <NumOfRepetitions>40</NumOfRepetitions>
        <StartingCycle>1</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>15</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>1</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>60.0</Temperature>
            <HoldTime>60</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
    </TCStage>
</TCProtocol>

[protocol.end]

[protocol.begin "stepone" "Fast SYBR Green with melt curve"]
<?xml version="1.0" encoding="UTF-8" standalone="yes"?>
<TCProtocol>
    <ProtocolName>Fast SYBR Green with melt curve</ProtocolName>
    <UserName/>
    <BlockID>0</BlockID>
    <TubeType>0</TubeType>
    <SampleVolume>20.0</SampleVolume>
    <RunMode>Fast</RunMode>
    <CoverTemperature>105.0</CoverTemperature>
    <Delay>0.0</Delay>
    <ExtendedPCRCycles>0</ExtendedPCRCycles>
    <ExtendedHoldTemp>0</ExtendedHoldTemp>
    <ExtendedHoldTime>0</ExtendedHoldTime>
    <TCStage>
        <StageFlag>PRE_CYCLING</StageFlag>
        <NumOfRepetitions>1</NumOfRepetitions>
        <StartingCycle>0</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>20</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
    </TCStage>
    <TCStage>
        <StageFlag>CYCLING</StageFlag>
        <NumOfRepetitions>40</NumOfRepetitions>
        <StartingCycle>0</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>3</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>1</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>60.0</Temperature>
            <HoldTime>30</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
    </TCStage>
    <TCStage>
        <StageFlag>DISSOCIATION</StageFlag>
        <NumOfRepetitions>1</NumOfRepetitions>
        <StartingCycle>0</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>15</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>60.0</Temperature>
            <HoldTime>60</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>1</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>15</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
            <CollectionMode>StepAndHold</CollectionMode>
            <Type>Dissociation</Type>
            <DeltaTemperature>0.3</DeltaTemperature>
        </TCStep>
    </TCStage>
</TCProtocol>

[protocol.end]


[protocol.begin "stepone" "Fast SYBR Green without melt curve"]
<?xml version="1.0" encoding="UTF-8" standalone="yes"?>
<TCProtocol>
    <ProtocolName>Fast SYBR Green without melt curve</ProtocolName>
    <UserName/>
    <BlockID>0</BlockID>
    <TubeType>0</TubeType>
    <SampleVolume>20.0</SampleVolume>
    <RunMode>Fast</RunMode>
    <CoverTemperature>105.0</CoverTemperature>
    <Delay>0.0</Delay>
    <ExtendedPCRCycles>0</ExtendedPCRCycles>
    <ExtendedHoldTemp>0</ExtendedHoldTemp>
    <ExtendedHoldTime>0</ExtendedHoldTime>
    <TCStage>
        <StageFlag>PRE_CYCLING</StageFlag>
        <NumOfRepetitions>1</NumOfRepetitions>
        <StartingCycle>0</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>20</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
    </TCStage>
    <TCStage>
        <StageFlag>CYCLING</StageFlag>
        <NumOfRepetitions>40</NumOfRepetitions>
        <StartingCycle>0</StartingCycle>
        <AutoDeltaEnabled>false</AutoDeltaEnabled>
        <TCStep>
            <CollectionFlag>0</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>95.0</Temperature>
            <HoldTime>3</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
        <TCStep>
            <CollectionFlag>1</CollectionFlag>
            <RampRate>100.0</RampRate>
            <Temperature>60.0</Temperature>
            <HoldTime>30</HoldTime>
            <ExtTemperature>0.0</ExtTemperature>
            <ExtHoldTime>0</ExtHoldTime>
        </TCStep>
    </TCStage>
</TCProtocol>

[protocol.end]



