PlusMinusWizardController.SETUP_GROUPING = Set Up
PlusMinusWizardController.ORDER_GROUPING = Order (Optional)

PlusMinusWizardController.CHEMISTRY_PAGE_TITLE = Methods & Materials
PlusMinusWizardController.CHEMISTRY_PAGE_HEADER = Define: Methods & Materials
PlusMinusWizardController.CHEMISTRY_PAGE_DESC = Review the reagents and type of template to use for this presence/absence experiment, then select the stages for the instrument run.
PlusMinusWizardController.CHEMISTRY_PAGE_TOOLTIP = Review the reagents and template type for this experiment, then select the run stages.

PlusMinusWizardController.PROBES_SETUP_PAGE_TITLE = Targets
PlusMinusWizardController.PROBES_SETUP_PAGE_HEADER = Set Up: Targets
PlusMinusWizardController.PROBES_SETUP_PAGE_DESC = Enter the name of the target to detect in the reaction plate (Unknown), then enter the name of the internal positive control (IPC) target.
PlusMinusWizardController.PROBES_SETUP_PAGE_TOOLTIP = Set up the target to detect and the IPC in the reaction plate.

PlusMinusWizardController.SAMPLES_SETUP_PAGE_TITLE = Samples & Replicates
PlusMinusWizardController.SAMPLES_SETUP_PAGE_HEADER = Set Up: Samples & Replicates
PlusMinusWizardController.SAMPLES_SETUP_PAGE_DESC = Enter the number of samples to test in the reaction plate, enter sample names, then enter the number of replicates for each type of reaction.
PlusMinusWizardController.SAMPLES_SETUP_PAGE_TOOLTIP = Set up the samples and replicates to test in the reaction plate.

PlusMinusWizardController.THERMAL_PROTOCOL_PAGE_TITLE = Run Method
PlusMinusWizardController.THERMAL_PROTOCOL_PAGE_HEADER = Set Up: Run Method
PlusMinusWizardController.THERMAL_PROTOCOL_PAGE_DESC = Review the reaction volume and the default thermal profile for the stages you selected for the instrument run. If needed, change your selection, edit the run method, or replace the run method with one from the library.
PlusMinusWizardController.THERMAL_PROTOCOL_PAGE_TOOLTIP = Review the instrument run conditions.

PlusMinusWizardController.REVIEW_PIPETTING_OUTPUTS_TITLE = Reaction Setup
PlusMinusWizardController.REVIEW_PIPETTING_OUTPUTS_HEADER = Set Up: Reaction Setup 
PlusMinusWizardController.REVIEW_PIPETTING_OUTPUTS_DESC = Select the assay type, then review the calculated volumes for preparing the PCR reactions. If needed, edit the reaction volume, excess samples, and/or component concentrations. Click "Print Reaction Setup" to print instructions on how to prepare the PCR reactions.
PlusMinusWizardController.REVIEW_PIPETTING_OUTPUTS_TOOLTIP = Review the components and volumes for the PCR reactions.

ChemistryAndSampleTypePage.CHEMISTRY_SELECTION = <HTML><font color="white">Use TaqMan\u00AE <A HREF=Help.chemistry>reagents</A> in the PCR reactions for this presence/absence experiment.</font></HTML>
ChemistryAndSampleTypePage.TAQMAN = TaqMan\u00AE Reagents
#next line also in messages_en_US.properties
ChemistryAndSampleTypePage.TAQMAN_DESC = The PCR reactions contain a forward primer, a reverse primer, and a TaqMan\u00AE probe. The primers are designed to amplify the target sequence. The TaqMan probe is designed to hybridise to the target sequence and generate fluorescence signal when the target sequence is amplified.
ChemistryAndSampleTypePage.CYCLING_SELECTION = <HTML><font color="white">Which <A HREF=Help.stages>stages</A> do you want to include in the instrument run?</HTML>
ChemistryAndSampleTypePage.TEMPLATE_SELECTION = <HTML><font color="white">Use a DNA <A HREF=Help.template>template</A> in the PCR reactions for this presence/absence experiment.</HTML>
ChemistryAndSampleTypePage.DNA = DNA
#next line also in messages_en_US.properties
ChemistryAndSampleTypePage.DNA_DESC = Use an optimized protocol to extract the DNA.Then, make sure that the A260/280 ratio is greater than 1.7, agarose gel electrophoresis shows the DNA is intact, and the DNA has not been heated above 60 \u00B0C.
ChemistryAndSampleTypePage.RAMP_SPEED_SELECTION = <HTML><font color="white">Use the standard <A HREF=Help.rampSpeed>ramp speed</A> for this presence/absence experiment.</font></HTML>
ChemistryAndSampleTypePage.STANDARD_DESC = For optimal results using the standard ramp speed, Applied Biosystems recommends standard reagents for your PCR reactions.
ChemistryAndSampleTypePage.FAST_DESC = For optimal results using the Fast ramp speed, Applied Biosystems recommends using Fast reagents for your real-time PCR reactions.

ChemistryAndSampleTypePage.CDNA = cDNA (complementary DNA)
ChemistryAndSampleTypePage.CDNA_DESC = You are adding cDNA to the real-time PCR reactions. You have already performed reverse transcription to convert the RNA to cDNA.

ChemistryAndSampleTypePage.RNA = RNA
ChemistryAndSampleTypePage.RNA_DESC = You are adding total RNA or mRNA to the real-time PCR reactions. Select "1-Step RT-PCR" perform reverse transcription (RT) and PCR in one instrument run or select "2-Step RT-PCR" to perform RT and PCR in 2 instrument runs.
ChemistryAndSampleTypePage.GDNA = gDNA (genomic DNA)
ChemistryAndSampleTypePage.GDNA_DESC = You are adding purified gDNA to the real-time PCR reactions. You have already extracted the gDNA from tissue or sample.\nUse an optimized protocol to extract the DNA. Then, make sure that the A260/280 ratio is greater than 1.7, agarose gel electrophoresis shows the DNA is intact, and the DNA has not been heated above 60 \u00B0C.


ChemistryAndSampleTypePage.UNPROCESSED_TISSUE_SAMPLE = Unprocessed Tissue/Sample
ChemistryAndSampleTypePage.UNPROCESSED_TISSUE_SAMPLE_DESC = You want to add unprocessed tissue or sample to the real-time PCR reactions. To proceed with this quantitation experiment, select a different template type OR extract DNA or RNA from the tissue or sample. Applied Biosystems recommends Applied Biosystems Purification Reagents.
ChemistryAndSampleTypePage.UNPROCESSED_TISSUE_SAMPLE_ERROR = <HTML><BODY><TABLE CELLSPACING=0 CELLPADDING=0 ><TR><TD>You selected unprocessed tissue/sample as your template.<br>To proceed with this quantitation experiment, select a different template type OR extract DNA or RNA from the tissue or sample. <br>To extract DNA or RNA from your tissue or sample, use Applied Biosystems <A HREF=Link.PurificationReagents>Purification Reagents</A>.</TD></TR></TABLE></BODY></HTML>

ChemistryAndSampleTypePage.WARNING = Warning

ChemistryAndSampleTypePage.SAMPLE_TYPE_SELECTION = <HTML><font color="white">What type of <A HREF=Help.template>template</A> do you want to use in the real-time PCR reactions?</font></HTML>

ChemistryAndSampleTypePage.RT_1_STEP = 1-Step RT-PCR
ChemistryAndSampleTypePage.RT_2_STEP = 2-Step RT-PCR

ChemistryAndSampleTypePage.TAQMAN_RT_2_STEP_INFO_TEXT = <HTML><BODY><TABLE CELLSPACING=0 CELLPADDING=0 ><TR><TD>You selected RNA as your template and to perform 2-step RT-PCR.<br>To proceed with this quantitation experiment, first perform reverse transcription to convert the RNA to cDNA, then select "cDNA" as the template. <br>Applied Biosystems recommends using the <A HREF=Link.HighCapacitycDNAArchiveKit.Taqman>High Capacity RNA-to-cDNA products</A>.</TD></TR></TABLE></BODY></HTML>

ProbesSetupPage.SETUP_PROBES = Set Up: Targets
ProbesSetupPage.UNKNOWN_TARGET_QUESTION = <HTML><BODY><TABLE CELLSPACING=0 CELLPADDING=0 ><TR><TD><font size=3 color=red><b>* </b></font>What is the name of the target you want to detect (<A HREF=Help.unknown>Unknown</A>)? The unknown target is detected using FAM reporter dye.</TD></TR></TABLE></BODY></HTML>
ProbesSetupPage.IPC_TARGET_QUESTION = <HTML><BODY><TABLE CELLSPACING=0 CELLPADDING=0 ><TR><TD><font size=3 color=red><b>* </b></font>What is the name of the <A HREF=Help.IPC>IPC</A> target? The IPC target is detected using VIC reporter dye.</TD></TR></TABLE></BODY></HTML>
ProbesSetupPage.ENTER_IPC = Enter IPC target name
ProbesSetupPage.NO_COLUMN=No column with index 
PreviewPanel.PREVIEW_ICON=This is a preview of the plate layout you are defining
ProbesSetupPage.ENTER_UNKNOWN = Enter Unknown target name
ProbesSetupPage.TARGET_TABLE_INFO_TEXT = Targets Used in Experiment
ProbesSetupPage.TARGET_NAME = Target Name
ProbesSetupPage.TASK = Task
PreviewPanel.PREVIEW=Well Preview
PreviewPanel.UNKNOWN=Unknown-IPC
PreviewPanel.AVAILABLE=Wells Needed/Available: 
PreviewPanel.EMPTY=Empty
   
SamplesSetupPage.SETUP_SAMPLES = Set Up: Samples & Replicates
SamplesSetupPage.SAMPLES_QN = <HTML><BODY><TABLE CELLSPACING=0 CELLPADDING=0 ><TR><TD><font size=3 color=red><b>* </b></font>How many <A HREF=Help.sample>samples</A> do you want to test in the reaction plate?</TD></TR></TABLE></BODY></HTML>
SamplesSetupPage.BIOGROUPS_QN = <HTML><BODY><TABLE CELLSPACING=0 CELLPADDING=0 ><TR><TD><font size=3 color=red><b>* </b></font>How many <A HREF=Help.sample>Biological Replicate Groups</A> do you need?</TD></TR></TABLE></BODY></HTML>
#next line also in messages_en_US.properties
SamplesSetupPage.SETUP_SAMPLE_DETAILS_INSTRUCTION = For each sample in the reaction plate, enter a sample name and select a sample colour.
SamplesSetupPage.ENTER_SAMPLE_NAME = Enter Sample Name
SamplesSetupPage.SAMPLE_NAME_ERROR=A sample name is required.
#next line also in messages_en_US.properties
SamplesSetupPage.SELECT_COLOR = Colour
SamplesSetupPage.UNKNOWN_IPC_QN = How many replicates do you need for reactions that contain sample? (Unknown-IPC wells)
SamplesSetupPage.NTC_IPC_QN = How many replicates do you need for reactions that contain IPC blocking agent instead of sample? (Negative Control-Blocked IPC wells)
SamplesSetupPage.NTC_NTC_QN = How many replicates do you need for reactions that contain water or buffer instead of sample? (Negative Control-IPC wells)  
SamplesSetupPage.NO_COLUMN=No column with index 
SamplePane.SAMPLE_NAME=Sample Name Required
SamplePane.CONCENTRATION=For concentration, enter a number from 0.000001 to 99999.99999.

SamplesSetupPage.BIOLOGICALGROUP_DEFINITION = <HTML><BODY><TABLE CELLSPACING=0 CELLPADDING=0 ><TR><TD><font color=white>Set Up <A HREF=Help.sampleTargetReactions>Biological Replicate Groups</A></font></TD></TR></TABLE></BODY></HTML>
SamplesSetupPage.NO_BIOLOGICAL_GROUPS = No Biological Replicates
SamplesSetupPage.SPECIFY_BIOGROUP = Specify Biological Replicate Groups

PMPlatePreview.ARRANGE_PLATE = Arrange Plate by:
PMPlatePreview.PLACE_NTCS = Place Negative Controls in:

WellCountPanel.WELL_COUNT = Well Count 
WellCountPanel.EMPTY_WELLS = Empty
WellCountPanel.UNKNOWN_IPC = Unknown-IPC
WellCountPanel.ILLEGAL_ARGUMENT=Orientation must be SwingConstants.VERTICAL or SwingConstants.HORIZONTAL
WellCountPanel.NTC_NTC = Negative Control-Blocked IPC
WellCountPanel.NTC_IPC = Negative Control-IPC
 
ThermalProtocolPage.INFO_TEXT = Note: Set the temperatures for the pre-PCR read and post-PCR read to the same value for accurate analysis.

ReactionPage.OTHER_CHEMISTRY_MESSAGE = The Reaction Setup screen is not available for "Other" reagents.
PlusMinusExperimentWizard.EXPERIMENT_DESC = Design a presence/absence experiment to determine whether a target nucleic acid sequence is present or absent in a sample.
PlusMinusExperimentWizard.DISPLAY_NAME = Presence / Absence

SamplePane.NO_COLUMN=No column with index 
PlatePreview.ILLEGAL_ARGUMENT=Illegal argument for PlatePreview
PreviewPanel.NTC=NC-NC
PreviewPanel.BLOCKED_IPC=NC-Blocked IPC
