PipettingReport.EXPERIMENT = Experiment:  
#PipettingReport.EXPERIMENT = \u4e2d\u6587:
PipettingReport.CREATED_BY = Created By:
PipettingReport.REACTION_SETUP_INSTRUCTIONS = Reaction Setup Instructions
PipettingReport.STEP_1 = 1. Prepare the samples.
PipettingReport.STEP_2 = 2. Prepare the standards.
PipettingReport.STEP_3 = 3. Prepare the reaction mix.
PipettingReport.STEP_4 = 4. Prepare the reactions.
PipettingReport.OVERVIEW = Overview   
PipettingReport.PAGE_OF = Page {0} of 
PipettingReport.REAGENTS = Reagents:
PipettingReport.TYPE = Type:

ReactionLayout_NTCTable_Wells.SECTION_HEADER = Pipette the negative control reactions to the appropriate wells.
ReactionLayout_NTCTable_Wells.NTC = Negative Control Reaction
ReactionLayout_NTCTable_Wells.ADD_TO_WELLS = Add {0} \u03BCL to Wells

ReactionLayout_NTCTable.SECTION_HEADER = Prepare negative control reactions for each target:
ReactionLayout_NTCTable.STEP_A = a. For each target, add the appropriate volumes of reaction mix and water to a tube.
ReactionLayout_NTCTable.STEP_B = b. Mix the reaction by gently pipetting up and down, then cap the tube.
ReactionLayout_NTCTable.STEP_C = c. Centrifuge the tube briefly to remove bubbles.

ReactionLayout_StandardTable_Wells.SECTION_HEADER = Pipette the standard reactions to the appropriate wells.
ReactionLayout_StandardTable_Wells.STANDARD = Standard Reaction
ReactionLayout_StandardTable_Wells.ADD_TO_WELLS = Add {0} \u03BCL to Wells

ReactionLayout_StandardTable.SECTION_HEADER = Prepare the standard reactions:
ReactionLayout_StandardTable.STEP_A = a. For each replicate group, add the appropriate volumes of reaction mix and standard to a tube.
ReactionLayout_StandardTable.STEP_B = b. Mix the reaction by gently pipetting up and down, then cap the tube.
ReactionLayout_StandardTable.STEP_C = c. Centrifuge the tube briefly to remove bubbles.
ReactionLayout_StandardTable.STANDARD_REACTION = Standard Reaction
ReactionLayout_StandardTable.REACTION_MIX = Reaction Mix
ReactionLayout_StandardTable.REACTION_MIX_VOL = Reaction Mix Volume
ReactionLayout_StandardTable.STANDARD = Standard
ReactionLayout_StandardTable.STANDARD_VOL = Standard Volume

ReactionLayout_UnknownTable_Wells.SECTION_HEADER = Pipette the unknown reactions to the appropriate wells.
ReactionLayout_UnknownTable_Wells.UNKNOWN_REACTION = Unknown Reaction
ReactionLayout_UnknownTable_Wells.ADD_TO_WELLS = Add {0} \u03BCL to Wells

ReactionLayout_UnknownTable.SECTION_HEADER = Prepare the reactions for the unknown:
ReactionLayout_UnknownTable.STEP_A = a. For each replicate group, add the appropriate volumes of reaction mix and sample to a tube.
ReactionLayout_UnknownTable.STEP_B = b. Mix the reactions by gently pipetting up and down, then cap the tube.
ReactionLayout_UnknownTable.STEP_C = c. Centrifuge the tube briefly to remove bubbles.
ReactionLayout_UnknownTable.UNKNOWN_REACTION = Unknown Reaction
ReactionLayout_UnknownTable.REACTION_MIX = Reaction Mix
ReactionLayout_UnknownTable.REACTION_MIX_VOL = Reaction Mix Volume 
ReactionLayout_UnknownTable.SAMPLE = Sample
ReactionLayout_UnknownTable.SAMPLE_VOL = Sample Volume 

ReactionLayoutSection.SECTION_HEADER = Prepare the Reactions
ReactionLayoutSection.INSTRUCTION = Use the plate layout and instructions to prepare the PCR reactions. Make sure the arrangement of PCR reactions matches the plate layout in the software.
ReactionLayoutSection.STEP_1 = Cover the reactions according to the plastic consumable you are using:
ReactionLayoutSection.STEP_1_A = a. Optical reaction plate: Seal the reaction plate with optical adhesive film.
ReactionLayoutSection.STEP_1_B = b. Tubes or tube strips: Cover the tubes with caps.
ReactionLayoutSection.STEP_2 = Centrifuge the plate or tubes briefly to remove bubbles.

ReactionMixSection.SECTION_HEADER = Prepare the Reaction Mix
ReactionMixSection.INSTRUCTION = Use the reaction table(s) and instructions to prepare the reaction mix for PCR reactions for each target assay. The reaction mix contains all components except sample, buffer, or standard.
ReactionMixSection.SUMMARY = Prepare the reaction mix for each target in separate tubes:
ReactionMixSection.STEP1 = 1
ReactionMixSection.STEP1_TEXT = Label an appropriately sized tube mix for the reaction mix. 
ReactionMixSection.STEP2 = 2
ReactionMixSection.STEP2_TEXT = Add the required volumes of each component into the tube.
ReactionMixSection.STEP3 = 3
ReactionMixSection.STEP3_TEXT = Mix the reaction mix by gently pipetting up and down, then cap the tube.
ReactionMixSection.STEP4 = 4
ReactionMixSection.STEP4_TEXT = Centrifuge the tube briefly to spin down the contents and eliminate air bubbles.
ReactionMixSection.STEP5 = 5
ReactionMixSection.STEP5_TEXT = Place the reaction mix(es) on ice until you prepare the reactions.
ReactionMixSection.LAST_STEP = Repeat steps 1 through 5 for each reaction mix in the experiment.

ReactionMixTable.TABLE_TITLE = {0} : Reaction Mix for {1} 
ReactionMixTable.COMPONENT = Component
ReactionMixTable.VOLUME_FOR = Volume (\u03BCL) for {0} Reactions (Plus Excess)
ReactionMixTable.MASTER_MIX = Master Mix ({0}X)
ReactionMixTable.ASSAY_MIX = Assay Mix ({0}X)
ReactionMixTable.TAQMAN_PROBE = TaqMan Probe
ReactionMixTable.FORWARD_PRIMER = Forward Primer
ReactionMixTable.REVERSE_PRIMER = Reverse Primer
ReactionMixTable.TOTAL_VOLUME = Total Reaction Mix Volume
ReactionMixTable.WATER = Water 

SamplesSection.SECTION_HEADER = Prepare the Samples
SamplesSection.INSTRUCTION = Use the sample dilution calculations table and instructions to dilute each sample to {0} {1} :
SamplesSection.TUBE = Tube
SamplesSection.SAMPLE_NAME = Sample Name
SamplesSection.STOCK_CONC = Stock Concentration of Sample ({0})
SamplesSection.VOLUME_DILUENT = Volume of Diluent to Add (\u03BCL)
SamplesSection.TOTAL_VOLUME = Total Volume of Diluted Sample (\u03BCL)
SamplesSection.VOLUME_STOCK = Volume of Stock to Add (\u03BCL)
SamplesSection.STEP1 = 1
SamplesSection.STEP1_TEXT = Label a separate microcentrifuge tube for each diluted sample.
SamplesSection.STEP2 = 2
SamplesSection.STEP2_TEXT = Add the required volume of diluent (buffer or water) to each empty tube as specified in the Volume of Diluent to Add (\u03BCL) column.
SamplesSection.STEP3 = 3
SamplesSection.STEP3_TEXT = Add the required volume of sample stock to each tube as specified in the Volume of Stock to Add (\u03BCL) column.
SamplesSection.STEP4 = 4
SamplesSection.STEP4_TEXT = Vortex each diluted sample for 3 to 5 seconds, then centrifuge the tube(s).
SamplesSection.STEP5 = 5
SamplesSection.STEP5_TEXT = Place the diluted sample(s) on ice until you prepare the reactions.

StandardDilutionSection.SECTION_HEADER = Prepare the Standards
StandardDilutionSection.INSTRUCTION = Use the standard dilution series table(s) and instructions to prepare the standards for the PCR reactions.
StandardDilutionSection.SUMMARY = Prepare the standard dilution series for each target separately:
StandardDilutionSection.STEP1 = 1
StandardDilutionSection.STEP1_TEXT = Label a separate microcentrifuge tube for each standard. For example, Std. 1, Std. 2, and so on.
StandardDilutionSection.STEP2 = 2
StandardDilutionSection.STEP2_TEXT = Add the required volume of diluent (buffer or water) to each empty tube as specified in the Volume of Diluent to Add (\u03BCL) column.
StandardDilutionSection.STEP3 = 3
StandardDilutionSection.STEP3_TEXT = Prepare Std. 1:
StandardDilutionSection.STEP3A = a
StandardDilutionSection.STEP3A_TEXT = Vortex the source (standard stock) for 3 to 5 seconds, then centrifuge the tube briefly.
StandardDilutionSection.STEP3B = b
StandardDilutionSection.STEP3B_TEXT = Using a new pipette tip, add the required volume of source (standard stock) to the tube for Std. 1. The required volume of source is specified in the Volume of Source to Add (\u03BCL) column.
StandardDilutionSection.STEP3C = c
StandardDilutionSection.STEP3C_TEXT = Vortex for Std. 1 for 3 to 5 seconds, then centrifuge the tube briefly.
StandardDilutionSection.STEP4 = 4
StandardDilutionSection.STEP4_TEXT = Prepare the remaining standards in the dilution series:
StandardDilutionSection.STEP4A = a
StandardDilutionSection.STEP4A_TEXT = Identify the source to use to prepare the standard (specified in the Source column).
StandardDilutionSection.STEP4B = b
StandardDilutionSection.STEP4B_TEXT = Using a new pipette tip, add the required volume of source to the tube for the standard. The required volume of source is specified in the Volume of Source to Add (\u03BCL) column.
StandardDilutionSection.STEP4C = c
StandardDilutionSection.STEP4C_TEXT = Vortex the standard for 3 to 5 seconds, then centrifuge the tube briefly.
StandardDilutionSection.STEP4D = d
StandardDilutionSection.STEP4D_TEXT = Repeat steps 4a through 4c to prepare each standard until you complete the dilution series.
StandardDilutionSection.STEP5 = 5
StandardDilutionSection.STEP5_TEXT = Place the standard on ice until you prepare the reactions.
StandardDilutionSection.LAST_STEP = Repeat steps 1 through 5 for each standard dilution series in the experiment.

